A holidic medium for Drosophila melanogaster

Matthew D. W. Piper, Eric Blanc, Ricardo Leitão-Gonçalves, Mingyao Yang, Xiaoli He, Nancy J. Linford, Matthew P. Hoddinott, Corinna Hopfen, George A. Soultoukis, Christine Niemeyer, Fiona Kerr, Scott D. Pletcher, Carlos Ribeiro, Linda Partridge

Research output: Contribution to journalArticle

Abstract

A critical requirement for research using model organisms is a well-defined and consistent diet. There is currently no complete chemically defined (holidic) diet available for Drosophila melanogaster. We describe a holidic medium that is equal in performance to an oligidic diet optimized for adult fecundity and lifespan. This holidic diet supports development over multiple generations but at a reduced rate. Over 7 years of experiments, the holidic diet yielded more consistent experimental outcomes than did oligidic food for egg laying by females. Nutrients and drugs were more available to flies in holidic medium and, similar to dietary restriction on oligidic food, amino acid dilution increased fly lifespan. We used this holidic medium to investigate amino acid–specific effects on food-choice behavior and report that folic acid from the microbiota is sufficient for Drosophila development.
Original languageEnglish
JournalNature Methods
Volume11
Issue number1
DOIs
Publication statusPublished - 1 Jan 2014

Fingerprint

Nutrition
Drosophila melanogaster
Diet
Food
Diptera
Choice Behavior
Formulated Food
Microbiota
Folic Acid
Drosophila
Ovum
Fertility
Nutrients
Dilution
Amino Acids
Research
Pharmaceutical Preparations
Experiments

Keywords

  • holidic diet
  • Drosophila
  • neuroscience

Cite this

Piper, M. D. W., Blanc, E., Leitão-Gonçalves, R., Yang, M., He, X., Linford, N. J., ... Partridge, L. (2014). A holidic medium for Drosophila melanogaster. Nature Methods, 11(1). https://doi.org/10.1038/nmeth.2731
Piper, Matthew D. W. ; Blanc, Eric ; Leitão-Gonçalves, Ricardo ; Yang, Mingyao ; He, Xiaoli ; Linford, Nancy J. ; Hoddinott, Matthew P. ; Hopfen, Corinna ; Soultoukis, George A. ; Niemeyer, Christine ; Kerr, Fiona ; Pletcher, Scott D. ; Ribeiro, Carlos ; Partridge, Linda. / A holidic medium for Drosophila melanogaster. In: Nature Methods. 2014 ; Vol. 11, No. 1.
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Piper, MDW, Blanc, E, Leitão-Gonçalves, R, Yang, M, He, X, Linford, NJ, Hoddinott, MP, Hopfen, C, Soultoukis, GA, Niemeyer, C, Kerr, F, Pletcher, SD, Ribeiro, C & Partridge, L 2014, 'A holidic medium for Drosophila melanogaster', Nature Methods, vol. 11, no. 1. https://doi.org/10.1038/nmeth.2731

A holidic medium for Drosophila melanogaster. / Piper, Matthew D. W.; Blanc, Eric; Leitão-Gonçalves, Ricardo; Yang, Mingyao; He, Xiaoli; Linford, Nancy J.; Hoddinott, Matthew P.; Hopfen, Corinna; Soultoukis, George A.; Niemeyer, Christine; Kerr, Fiona; Pletcher, Scott D.; Ribeiro, Carlos; Partridge, Linda.

In: Nature Methods, Vol. 11, No. 1, 01.01.2014.

Research output: Contribution to journalArticle

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T1 - A holidic medium for Drosophila melanogaster

AU - Piper, Matthew D. W.

AU - Blanc, Eric

AU - Leitão-Gonçalves, Ricardo

AU - Yang, Mingyao

AU - He, Xiaoli

AU - Linford, Nancy J.

AU - Hoddinott, Matthew P.

AU - Hopfen, Corinna

AU - Soultoukis, George A.

AU - Niemeyer, Christine

AU - Kerr, Fiona

AU - Pletcher, Scott D.

AU - Ribeiro, Carlos

AU - Partridge, Linda

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Piper MDW, Blanc E, Leitão-Gonçalves R, Yang M, He X, Linford NJ et al. A holidic medium for Drosophila melanogaster. Nature Methods. 2014 Jan 1;11(1). https://doi.org/10.1038/nmeth.2731