The aim of this study was to compare the outcome measures from two different methods for estimating the coefficient of variation (COV) in cell area for human corneal endothelia. A single non-contact specular micrograph was obtained from the central region of the corneal endothelium of 100 healthy non-contact lens-wearing white European subjects, aged from 32 to 62 years. The captured image file was either assessed using a machine-based algorithm in which 21 cells were marked and their areas reported (designated as the TOPCON ‘centre-dot’ method), or by an overlay and semi-automated computer-based image analysis system of the entire image of around 200 cells (designated as the AUTO method). The average cell area values were used to calculate the endothelial cell density (ECD), while the COV was calculated from the standard deviation of the cell area measures.
- vision sciences